Primer

Part:BBa_K4508000:Design

Designed by: Julian Chen   Group: iGEM22_City_of_London_UK   (2022-10-04)


HSP65 Forward Primer (M.smegmatis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers designed on Benchling were calibrated to PCR


Source

Designed by the City of London iGEM 2022 Team on Benchling. Primers were constructed from the HSP65 gene of M.smegmatis strain: ATCC19420

References